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At 7 days postfertilization (dpf), after having undergone completion of brain formation at 5 dpf, zebrafish exhibit behavioral, electrographic, and molecular changes to chemical convulsants that would be expected from a rodent seizure model.
We formulated a mixture of organochlorine pesticides, PCBs, and PBDEs in defined proportion to the single component (p,p′-DDE) that we previously determined to enhance chemical-induced seizures after completion of brain development.
We previously reported that exposure to DDT or its metabolite DDE during zebrafish neurodevelopment enhances seizure behavior response to the algal toxin domoic acid after completion of brain maturation, a likely exposure scenario for the CSL population of the Channel Islands (Tiedeken and Ramsdell 2009).
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I blame it on the endless left brain edits I had to make after the completion of a lengthy right brain novel.
Now they can save themselves the trouble, thanks to the completion of a Web-based brain atlas announced here today.
If necessary, mice were euthanized prior to the completion of the study when brain tumors became peripherally ulcerated or when mice showed morbidity as defined by our Institutional Animal Care and Use Committee guidelines.
The timeframe considered was from the diagnosis of brain death until completion of the organ recovery procedure.
After completion of experiments, fresh-frozen brains were cryostat-cut into coronal 20 µm sections for further validation of the extent of lesion using Cresyl violet staining, as previously described [9], [10].
Two-three weeks after completion of tamoxifen treatment, whole brains were dissected out immediately after anesthesia with sodium pentobarbital (0.07 mg/g body weight), and HPLC samples were made according to methods described previously [5].
After the completion of the experiments, the brains of all mice were perfused and fixed.
Upon completion of the experiments, the rats' brains were removed under deep anesthesia induced by pentobarbital (31 mg/kg, i.p).
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