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After completion of all experiments and termination of first run, the second run was carried out with a new mud with a new viscosity (higher viscosity).
After completion of all experiments, basic analytic operations are used to collect and summarize experimental data.
After the completion of all experiments (see below) one particularly interesting isolate was identified by DSMZ (German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany).
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VY performed the endotoxin experiments and oversaw the completion of all other experiments described in this manuscript.
All other running procedures were as previously described for Experiments 1 and 2. At the completion of all three experiments, rats were deeply anaesthetised using sodium pentobarbital (60 mg/kg, i.p.; Euthatal; Merial Animal Health, Harlow, UK), then transcardially perfused with 0.1 M phosphate-buffered saline (PBS) followed by 4% paraformaldehyde in 0.1 M PBS PFAA).
After each run, the experimental setup was cleaned with distilled water, and after completion of all the experiments, it was cleaned thoroughly with 0.1 (N) of nitric acid solution, then 0.1 (N) of sodium hydroxide solution followed by washing with deionized water.
Upon completion of the experiments, all mice were injected with a lethal dose of pentobarbital (Nembutal, 100 mg/kg, i. p).. Brains were frozen, and cut into 20-µm sections using a cryostat (CM 3050S, Leica, Germany).
Upon completion of the experiments, all rats were weighed and blood was collected from the anesthetized animals into blood collection tubes after a 12-h overnight fast.
After the completion of the experiments, the brains of all mice were perfused and fixed.
At the completion of the experiment, all of the animals were killed with overdose of pentobarbital.
After the completion of the experiment, all animals were returned to their home cage and allowed to recover overnight prior to all +24 hour experimentation.
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CEO of Professional Science Editing for Scientists @ prosciediting.com