Primary outcomes were completion of a standardized protocol and the length of time needed to complete all steps.
However, uracil N-glycosylase (UNG) is an enzyme that has a degree of thermostability and may also become reactivated after completion of a PCR protocol [ 29, 30].
Response assessment following completion of a therapy protocol is equally as vital as it can avoid excessive and often more invasive treatments and allow for proven consolidation treatments.
While bone geometry in the cortical diaphysis was not significantly different between accelerated limbs and their contralateral controls at completion of the protocol, a longitudinal analysis of the in vivo microCT scans between baseline and 3wk showed that cortical area in CTR tibiae decreased significantly by 2% (p = 0.009) during the experimental period.
One patient in the CE arm was found to have LD after the completion of protocol chemotherapy due to protocol violation, and this patient was considered ineligible.
Cows inseminated at completion of the protocol have a longer voluntary waiting period, which could potentially extend their lactation length, allowing them to recover BCS at the end of their lactation and ultimately impacting their subsequent lactation.
For further details, see Table 4. Thirteen patients (43 %) had a normal completion of the protocol consisting of CR or CRi (Fig. 1).
We excluded individuals with conditions or treatments that would interfere with participation or completion of the protocol such as an underlying disease likely to limit life span or increase the risk of the intervention, or that had a confounding effect on the outcomes of the study, such as medication or a disease related to metabolism such as Cushing's syndrome.
As the analysis was done on a paired basis completion of the protocol was necessary, hence the analysis is per protocol, glucosamine n = 41, reparagen n = 38.
