Participants were given a self-completion diet, health and lifestyle questionnaire at the clinic; this included questions on body size and weight in stones/pounds, height in feet/inches, and inside leg measurement in inches.
Participants were mailed kits containing three questionnaires for self-completion (diet, family history, and use of personal care products), written consent documents, and support information for telephone interviews and home visits.
The time between completion of diet and follow-up visit was 24 ± 6 months (range, 17 36).
One week prior to the completion of the diet regimens, the different experimental groups were implanted with telemetry probes for BP recording.
Exclusion criteria are factors that either interfere with endogenous sex hormone levels or successful completion of the diet or exercise intervention (see Table 1).
Subjects first underwent baseline assessments, including a 3-day diet diary, completion of the Three Factor Eating Inventory [20], measurements of resting metabolic rate (RMR) by hood indirect calorimetry (2900 metabolic cart, Sensormedics, Yorba Linda, CA), and body composition measurement by dual-energy x-ray absorptiometry (DPX whole-body scanner, Lunar Radiation Corp., Madison, WI).
After completion of adaptive training, diet was restricted to 85% of ad libitum daily consumption by weight.
This diet permits completion of development, albeit delayed by 2 4 days, and gives rise to smaller-than-normal adult flies (Layalle et al., 2008).
Although the completion of a duplicate diet study is more labor intensive, it results in a more detailed and personalized view per volunteer regarding the HBCD exposure compared with a market basket study.
To rule out food responsive symmetrical onychomadesis all the dogs were fed their original diet after completion of the trial, and none of the owners reported any recurrence of symmetrical onychomadesis within the first month.
All participants received training on diet-diary completion during this measurement session when participants were asked to recall their last meal.
