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To assess completeness of the draft genomes, we determined the number and size of contigs.
Thus, increasing the accuracy and completeness of the draft genomes of early branching metazoans is an important step in improving their utility for future evolutionary and functional studies aimed at unravelling the origin of animal multicellularity.
We used three methods to assess the completeness of the draft Stylonychia macronuclear genome assembly: 1) The percentage of reads mapping to the assembly, 2) the percentage of conserved core eukaryotic genes (CEGs) (Parra et al. 2007) with Stylonychia homologs, and 3) whether a complete set of tRNA genes was predicted.
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To determine the completeness of the Epulopiscium draft genome, we analyzed the 92 large contigs using two approaches.
Evaluation of the completeness of the V. macrocarpon draft genome assembly was done using CEGMA [ 24, 25] and showed that 377 (82%) out of the 458 Core Eukaryotic Genes (CEG) were present in the assembly.
The full and partial completeness of the bowhead whale draft genome assembly was evaluated as 93.15% and 97.18%, respectively, by the CEGMA pipeline (Parra et al., 2007), which is comparable to the minke whale genome assembly (Yim et al., 2014).
We used a new analysis tool, CheckM (Parks et al., PeerJ, 2015) to estimate the completeness of our draft genomes, which is between 90.7 to 97.7%.
We used CheckM to evaluate the completeness of our draft genomes with a set of single-copy marker genes that are specific to proteobacteria (lineage-specific marker set of CheckM p_Proteobacteria, UID3880) (Parks et al., 2015).
Stage II consisted of cognitive interviews to assess completeness, comprehension, and interpretability of the draft Flu-PRO instrument from the respondent's perspective.
Completeness of the gene space in the draft was assessed using CEGMA 2.3 (Parra et al. 2007, 2009).
Appropriateness, clarity and completeness of the items in a draft version of the instrument was revised by experts who also judged the appearance, question sequence and completion time.
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