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The sequence completeness of the assembly was estimated with CEGMA software [ 36] and TargetIdentifier [ 37].
Completeness of the assembly was assessed using two sets of information from the 10 bee genomes.
However, these decreases in continuity are accompanied by an overall improvement in accuracy and completeness of the assembly.
To assess the completeness of the assembly, reads from short-insert size libraries were re-mapped onto the assembly.
Completeness of the assembly was confirmed by the successful mapping of up to 97% of reads from short insert libraries.
As expected, the completeness of the assembly is correlated with the sequencing depth (or expression level) of each gene.
Similar(43)
In order to test completeness of the assemblies, the CEGMA Core Eukaryotic Genes Mapping Approachh) pipeline [ 60, 61] was used.
To test the completeness of the assemblies, we tested for the presence of highly conserved genes from other species in two ways.
To assess the completeness of the assemblies we ran CEGMA [ 28], which scores the presence of 248 core eukaryotic genes (CEGs) in a given assembly.
Completeness of the assemblies was assessed by mapping all reads from each tissue library back to their respective de novo assembly (per species) individually with Bowtie and TopHat [ 46].
CEGMA or Core Eukaryotic Genes Mapping Approach [ 43], a pipeline used to accurately annotate core genes in eukaryotic genomes, was also searched to determine the quality and completeness of the assemblies.
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