After two cycles of washing and centrifugation, the cells were resuspended with 2 mL PBS and disrupted completely by vigorous sonication.
Also remove completely any overly vigorous, upright shoots not needed to create permanent branches.
After maturation, expanded cumulus cells and corona cells were completely removed by vigorous pipetting of the COCs in the presence of 0.1% hyaluronidase for 1 to 2 min.
There is also the stumbling conversation of the other blind novitiates, but we are all really dependent on the voice of our guide, Romeo Edmead, a vigorous, charming man, completely unruffled by the tumult we find around us.
There is also the stumbling conversation of the other blind novitiates, but we are all really dependent on the voice of our guide, Romeo Edmead, a vigorous, charming man, completely nonplussed by the tumult we find around us. "Nonplused" (that's our preferred spelling, by the way) means "bewildered" or "dumbfounded".
The samples were incubated at 100°C for 10 minutes, or until the pellet is completely dissolved, by intermittent and vigorous mixing.
We found that 51 trials were necessary for the CN neuron to start eliciting a vigorous CR that completely suppressed spike generation by the IO neuron, whereas 19 trials were sufficient in the normal case.
"No one can expect his views to be free from vigorous challenge, but all must feel completely safe in speaking out".
More vigorous hydrothermal activity can completely bury the underlying lava flows.
In the present case, 220 μL polyallylamine (Sigma-Aldrich) was completely dissolved in distilled water under vigorous stirring at 70 °C.
"It is a vigorous report that, while remaining completely unpatronising, nevertheless makes deeply uncomfortable reading.
