Sentence examples for completely denatured from inspiring English sources

The GC-clamp, which is a sequence that is rich in guanine and cytosine, is added to the 5′ end of the forward primer in order to prevent DNA from being completely denatured into single strands and to improve band resolution in denaturing gels.

Upon heating, holo-LmCld at pH 4.0 is completely denatured with a broad transition (Tm = 35 °C) that is completed at approximately 50 °C, exhibiting complete loss of any ellipticity.

His life has been completely denatured by this duplicity.

In contrast, the unprotected protein was completely denatured and lost its antibody binding capacity.

Beyond 77 °C (170 °F) proteins begin to lose structural integrity (i.e., they are completely denatured) and tenderness begins to improve.

Consistent with this, intact tissue was only minimally denatured by treatment at 85°C for 15 min, whereas excised tissue was completely denatured by this protocol.

The polypeptide assumes a fully folded antiparallel β-sheet/turn structure at room temperature, and yet is completely and reversibly denatured at 125°C, adopting a predominant polyproline II conformation.

B samples represent the background fluorescence observed when DNA is denatured completely.

Two attempts were made to purify nNOS with pterin-free buffer or the buffer supplemented with 1, but the protein denatured completely upon trypsinolysis required for generating the heme domain.

To test this hypothesis, the completely reduced species was denatured by the addition of urea from a side arm of the anaerobic cuvette.

After transcription, RNA samples were heat denatured and purified by using urea-denaturing polyacrylamide gels.