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Reverse transcription was completed using High capacity cDNA archive kit (Applied Biosystem, California, USA).
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Determinations of analytes were completed using high-performance liquid chromatography (HPLC) with UV detection.
Standard polymerase chain reaction (PCR) was completed using High-fidelity ExTaq DNA polymerase (TaKaRa Biotechnology (Dalian) Co., Ltd., China).
GSTP1 Ile105Val (rs1695) and GSTA1 (three linked based substitutions in promoter at -567, -69 and -52) genotyping were completed using high-throughput MALDI-TOF [ 24, 25].
Once the number of cycles was identified, 16 PCRs (50 µL) were completed using high-fidelity Taq DNA polymerase (Clontech), and the products were cleaned using a QiaQuick column (QIAGEN).
Numerical simulations are completed using the high-order spectral difference (SD) method.
cDNA synthesis was completed using a high-capacity cDNA reverse transcription kit (Applied Biosystems, Foster City, CA, USA).
Measurement of sE-selectin was completed using a high-sensitivity ELISA assay (Parameter Human sE-Selectin Immunoassay, R&D Systems).
Experiments in cell culture were completed using a high-resolution NMR system, where line shape and the small coil volume allowed optimal shimming conditions and rapid detection of metabolism.
Single-phase high-pH corefloods were completed using Berea sandstone and reservoir samples to calibrate and validate geochemical simulations.
High-throughput sequencing of libraries was completed using standard methodsas described by Yang et al. [ 61].
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