Sentence examples for completed sequences were from inspiring English sources
A total of 381 completed sequences were obtained from the National Institute of Biotechnological Information (NCBI, http://www.ncbi.nlm.nih.gov/genomes/lproks.cgi, accessed: 10/20/2006) and an additional 88 draft genomes were obtained from the Integrated Microbial Genomes database [43] (http://img.jgi.doe.gov/cgi-bin/pub/main.cgi, accessed: 10/20/2006).
Completed sequences were compared with the non-redundant GenBank+ EMBL + DDBJ database using BLASTN and BLASTX homology search software.
The completeness of each sequence can vary – some completed sequences are more "complete" than others.
The amplified fragment was cloned, and the complete sequences were determined.
Both RACE amplified 5′ and 3′ ends of PhaNPR1 were cloned, and the complete sequences were determined.
The amplicons were sequenced twice in each direction, and complete sequences were obtained by assembling overlapping contigs with DNASTAR DNASTARR, Inc., Madison, Wisconsin, USA).
Complete sequences were assembled using Sequencher 4.10.1, and 18 monomers were identified that showed sequence variation relative to one another (Table 1).
In the second cloacal swab-virus isolate pair, complete sequences were obtained from the virus isolate, whereas only 15 influenza sequence reads were obtained from the cloacal swab.
Complete sequences were reconstructed by overlapping shorter sequences, allowing us to verify that nucleotide changes were not attributable to RT-PCR artefacts.
Ages for HVSI of complete sequences were also independently calculated.
Complete sequences were assembled using Seqman II (DNASTAR).
