Sentence examples for completed replication from inspiring English sources
The majority of chromosomes that had completed replication, as judged by incorporation of biotin dUTP, had paired sister chromatids.
After growth at 42°C for 100 min, >90% of cells lacked Ssb or DnaQ foci and had completed replication, as judged by flow cytometry.
These are cells that have entered mitosis without having completed replication, most likely due to the compromised G2/M checkpoint in the absence of Wee1 and/or Chk1.
Conversely, within the sml1 rad53 set of strains, only sml1Δ rad53Δ pif1-m2 rrm3Δ cells almost completed replication by 90 min.
Our results were consistent with the interpretation that the overlap of IF signals from these three proteins with EdU decreases as replication proceeds across a chromatin fiber, and regions that have completed replication lack these proteins all together.
Cell populations lacking a replisome focus (19%27%%; depending on the replisome component) were enriched for shorter cells (<3.5 μm long) that had presumably not yet initiated replication and longer cells (>4.5 μm long), which were expected to have completed replication (G2).
The length of the sequence also affects replicability through the time required for completing replication.
This did not prevent the cluster from completing replication because the leftward fork initiated at a map position of 240 kb replicated all the DNA up to the stalled fork.
The chromatin condenses prematurely in the G2 phase of the cell cycle, shortly after completing replication.
Loading of DnaC by primosomal proteins occurs at arrested replication forks [5] and in the dnaC2 mutant Maisnier-Patin et al [7] showed that if active DnaC is not available during elongation about 18% of the cells fail to complete replication.
It appears that this is a dynamic process by which cancer cells are prevented from completing replication over several days in the animal, perhaps accounting for the very modest effects of the drug combination in short term culture in vitro.
