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Pour salt into the completed mixture and continue stirring.
Pour the completed mixture into the pan or pans.
Pour the completed mixture into the storage containers.
The completed mixture should have the appearance of oatmeal.
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The agar-cell mixture was plated on top of a bottom layer with 1% agar completed medium mixture.
After addition was completed, the mixture was stirred for additional 30 min to remove all HCl.
After the addition was completed, the mixture was stirred for additional 30 min to remove all HCl.
After the stirring was completed, the mixture was centrifuged at 4,500 rpm to separate the capped gold nanoparticles.
Once the reaction was completed, the mixture was diluted with water (30 mL) and passed through a Waters Sep-Pak tC18 Plus Long SPE cartridge (WAT036800).
After the digestion was completed, the mixture was allowed to cool for 30 min and the TRN was determined by with a Kjeltec auto 2300 analyser.
After the reaction completed, the mixture was transferred to a rotary evaporator to separate the water and any solvent that remained.
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