Sentence examples for completed medium from inspiring English sources
RBcs was prepared by seeding 10-cm dish (Falcon; BD Bioscience, Franklin Lakes, NJ, USA) with 1 × 107 RB cell in 10 mL of completed medium for 24 h and centrifuged to remove cell debris.
TF-1 cells were cultured in RPMI1640 completed medium with 2 ng/ml human IL-3 (PeproTech, Rocky Hill, NJ).
Green clones in the medium containing 2 µg/ml puromycin (Invitrogen) were identified by microscopy, and were picked and cultured in completed medium with puromycin for one week.
Briefly, KB cells were initially incubated in completed medium containing 5 nM of BPR0L075 that yielded 40% cell survival for a period of 3∼4 weeks, and the cells that proliferated were repeatedly subcultured in completed medium containing increasing concentrations of the drug (at 20% increment each time).
Isolated fibroblast-like synoviocytes (FLSs) were cultured in completed medium in 75-mm tissue culture flasks.
Cells were again fed with EGM-2MV comediumd medium after overnight incubation with the transfection mix.
200 μL complete medium was added to the lower chamber.
Leu+ transformants were selected on SC medium (synthetic complete medium) lacking leucine (SC-Leu).
MSCs isolated from amnion were cultured in complete medium similar to BM-MSCs.
Then, complete medium was added.
