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All measurements were completed in duplicate using mitochondria (0.5 mg mitochondrial protein/ml) in incubation medium (145 mM KCl, 30 mM Hepes, 5 mM KH2PO4, 3 mM MgCl2, 0.1 mM EGTA, 5 µM rotenone, and 0.4 µg nigericin/mg mitochondrial protein, pH 7.4).
All samples were completed in duplicate.
These were all completed in duplicate.
Inclusion/exclusion decisions and data extraction were completed in duplicate.
Systematic screening protocol with all steps completed in duplicate.
Each comparison was completed in duplicate and with a dye flip.
Similar(45)
Experimental values represent at least three different reaction experiments completed in duplicates.
Briefly, 1 × 106 cells were seeded onto 60 mm cell culture plates in 2 ml completed medium in duplicate.
This article was conducted following rigorous systematic review methodology; 11 databases were searched, not limited by language or publication type, and study selection, data extraction and quality assessment were all completed independently, in duplicate.
All biomass carbohydrates were completed in single analysis with duplicate tests of a known composition control (e.g. debarked and clean Southern Loblolly pine chip sample) to assure the accuracy of the analysis in Weyerhaeuser's analytical group.
Medline and manual searches were done (completed independently and in duplicate) to identify all published (manuscripts and abstracts) randomized controlled trials (RCTs) that comparing adjuvant trastuzumab treatment for HER2-positive early breast cancer to observation.
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