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This technique has the advantage of being able to measure the fingers of completed cells, and this also explores the error introduced by measuring the resistance across interjacent fingers.
When the fermentation was completed, cells were pelletized and ultrasonicated by a probe VCX800 system (Sonics, Newtown, USA).
Once treatment was completed, cells were trypsinized and stained with 1 µM NAO at room temperature in the dark for 10 min, followed by FACS analysis.
The DSST, a test of psychomotor speed (27), was measured as the number of correctly completed cells.
After the exposure completed, cells were harvested in ice-cold phosphate buffer saline by scraping and washed with phosphate buffer saline at 4 °C.
Once the incubation time was completed, cells were washed with PBS and stained for 30 minutes at 37°C in CO2 incubator with either 100 nM mitotracker green or 100 nM DiOC6 (both from Molecular Probes, Invitrogen) in respective wells.
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VU completed cell culture experiments.
At 24 h the numbers had roughly doubled, reflecting a completed cell cycle.
CGJ completed cell culture experiments, data analysis, real-time PCR and drafted the manuscript.
Detection was performed so faster than the time required to complete cells mitosis.
This zone of ablation correlated to complete cell destruction below 25 °C in in vivo-like tissue.
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