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The partial sequence of CcGCC1 cDNA annotated in clone [GenBank: CX287481] was completed by sequencing its 3' end.
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All regions of the genes were covered and no coverage gaps had to be completed by Sanger sequencing.
Sequencing data were produced following a chromosome map-based approach and classical shotgun strategy completed by end sequencing of inserts from a miniBAC library.
These analyses were completed by a sequencing service (Fraunhofer Institute for Molecular Biology and Applied Ecology, Aachen, Germany) using the above mentioned primers and additionally zcyt-1R (5′-gtttaagccaagggggttgt-3′) and zcyt-2F (5′-ctcgattctttgccttccac-3′) to obtain two overlapping fragments.
Usually, it was still possible to obtain a complete sequence by sequencing from 5' and 3' ends such that the sequences only rarely remained incomplete after extended sequencing efforts.
However, all genomic regions tested were represented, at least to some extent, and it was feasible to complete genomes simply by sequencing to a sufficient depth [ 9].
Gap closure was completed by Sanger-based sequencing of select regions to bridge contigs.
DNA sequences were completed by using the Lasergene sequence analysis software package (DNAStar, Madison, WI, USA).
The sense of removal from the real world was completed by a title sequence that showed soulful helicopter shots of the Irish landscape accompanied by Neil Hannon of the Divine Comedy's lachrymose Songs of Love.
GO analysis was completed by submitting gene sequences online (see Materials and Methods).
Identification of putative InR gene orthologs was completed by multiple protein sequence alignments followed by phylogenetic analysis.
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