Finally, NER is completed by gap-filling DNA synthesis, followed by strand ligation [45].
The NER reaction involves adduct recognition, adduct cleavage via dual incision, damaged fragment unwinding, and is completed by gap-filling DNA synthesis [ 14, 15].
Gap closure was completed by amplification of gap regions by PCR followed by Sanger sequencing of the products.
These draft models were then completed by automated and manual gap filling analysis and validated against 260 FBA-based tests.
The gaps were completed by other adjacent primers.
Gap closure was completed by Sanger-based sequencing of select regions to bridge contigs.
Assemblies 2-15 were generated with the Celera Assembler [ 33]. excluding gaps The sequence completed by BCM-HGSC (Genbank: AC204781.3) was assembled using Phrap [ 40] and Atlas [ 41].
All regions of the genes were covered and no coverage gaps had to be completed by Sanger sequencing.
Further species-specific primers were designed as required for each genome in order to cover remaining sequence gaps; the complete genomes were completed by primer walking.
After the Astoria Megler Bridge was completed on 27 August 1966, SR 401 was extended west to the new bridge and the gap between Megler and Knappton was completed by 1968.
Formation of new epidermis over the dermal gap of < 2 cm is usually completed by days 10 to12, concomitant to granulation tissue formation.
