After the assay was completed, viable cells in each well were counted using a Vi-Cell XR cell counter and the cell counts used to normalize the OCR rates, with OCR being expressed as pmoles oxygen/minute/10 cells.
"Normal Girl" shows signs of turning into a coherent novel, complete with viable characters and telling details, upon Miranda's retreat to Minnesota (a k a Minne-sober) for a lifestyle overhaul.
It was realized that, through the process of differentiation, adult mammalian cells lose totipotency the ability to become any of the different cell types required for making a complete and viable animal.
When book number two was in its nascent stages I happily rhapsodized about it every chance I got, and now that I'm closer to completing a viable draft that could conceivably be read, and judged, by other people, I break out in a cold sweat just thinking about it, forget talking about it!
These experiments were used to optimise the system and verify that the complete system is viable under different conditions, styles and swimmers.
Genes required for growth in different environmental conditions were identified in two systematic genetic screens [30], [31] using the complete set of viable haploid gene deletion strains [1].
After 72 h of culture in 10,000 ng/ml of mycolactone there was a complete elimination of viable, WST-1 reactive cells in the cultures (Figures 1B and 1D).
The culture-adapted serovar Bataviae and Lai strains had completely lost their virulent phenotype as displayed by failure to induce weight loss or pathology and by complete clearance of viable bacteria of these strains in the guinea pig infection model (Figure 1).
A fully functional GFP-tagged chimera of the protein Pot1p, a thiolase localized to the peroxisomal matrix, was introduced into an arrayed library containing the complete collection of viable yeast deletion mutant strains (∼4000 strains after quality control selection - see Materials and Methods).
