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A total of 5 × 10 CLL cells were stained with fixable viability dye (ebioscience) to exclude apoptotic cells, resuspended in 1 mL RPMI complete, transferred to coated cell culture slides and incubated for 1 h at 37°C.
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∼1200 DPM represents complete transfer to the 10 nmol of substrate present.
Licence holders had until 15 January of the subsequent year to complete transfers to cover any overruns of their quotas.
While recognising advantages to GP follow-up, there was a stronger level of support for shared care between the GP and a specialist, rather than a complete transfer to GP led care (Brennan et al, 2011a).
She underwent her own refit later in 1915 and once completed, was transferred to the Suez Canal Patrol.
The complete assembly was transferred to a cabinet maintained at 37°C for 6 hours.
The complete fluid was transferred to the 1.5-ml sample tube.
The complete slurry was transferred to chromatography column and the supernatant with the unbound proteins was removed.
Renown rejoined Home Fleet once those missions were completed, but was transferred to Force H in October 1942 to participate in Operation Torch.
After fitting-out was completed she was transferred to Kiel, where she was commissioned for sea trials.
Four days after mate trials were completed; females were transferred to new vials supplemented with yeast.
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