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The city allows property owners to take years, and depending on the building type, decades to complete the retrofitting process, however.
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Transfection of the retrofitting plasmids and retrofitted BACs with PEI22 showed that the luciferase was efficiently expressed.
This new monolithic aerogel window is then used in the retrofitting project of an educational building completed in the 1960s.
He said the manufacturer's assurances had convinced them to go ahead with the retrofitting.
Alternatively the Cre can be expressed from a separate plasmid that is co-transfected with the retrofitting plasmid, and then lost once retrofitting has occurred.
In one system, the Cre protein is expressed from the retrofitting plasmid itself in vivo.
The retrofitting plasmids give almost identical luciferase expression to the control pCMV-LUC and pCIKLux plasmids.
The retrofitting is expected to complete in Fall of 2012 at a cost of $5.7 million US dollars.
In 1998, the International Union of Railways (UIC) agreed on an Action Plan, proposing the retrofitting of the complete European rail freight fleet.
The retrofitting of the E-3 squadrons were completed in December 2000.
The first retrofitting step allowed the insertion of Hprt homologous recombination targeting arms as we have described previously [ 45].
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