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The complete reassignment of codons to unnatural amino acids at one or two non-adjacent sites per protein has already found wide utility (see other papers in this volume).
Complete reassignment of a stop codon is precluded by release factors (RFs), which recognize stop codons to terminate translation.
However, for complete reassignment of UAG to a sense codon, a necessary and critical step is to knock out RF1 from the E. coli genome.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com