So far, attempts to uncover PU.1's role in HSC biology have failed because of the technical limitations of complete loss-of-function models.
Hence, inhibition of translation is transient and may not result in a complete loss-of-function.
To test theses hypotheses, we generated and analyzed phenotypes associated with a complete loss-of-function allele of Toll-9.
Alternatively, it is also possible that the Chk1D469G mutant is simply a partial, rather than complete, loss-of-function mutant.
In order to study Toll-9 receptor function, we generated a complete loss-of-function allele by targeted homologous recombination [44].
The MEGF10 EGFP fusion construct was introduced as extra-chromosomal array into ced-1 complete loss-of-function mutants ced-1 e1735) ced-1 e1735e wild-type bandground.
Although one small (26 bp) LKB1 deletion representing a complete loss-of-function was identified by sequencing (Table S1, Figure 1A), larger deletions would have been missed.
Ideally, complete loss-of-function mutants need to dispose of entire coding sequences.
However, for most of them, the complete loss-of-function feature has not been demonstrated.
Complete loss-of-function of DFR2 gene leads to near white flowers.
