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The ion peak of the non-labeled peptide was not detected at an m/z of 966.4 Da in both the light and intermediate dimethyl-labeled sample spectra, indicating complete labeling of the peptide.
For miRNA profiling, 100 ng and 20 ng of cellular and extracellular total RNAs, respectively, were fluorescence-labeled using the miRNA Complete Labeling Reagent and Hyb Kit (Agilent Technologies) according to manufacture's protocol (Agilent miRNA microarrays version 2.2).
Each array was hybridised with 100 ng of Cy3-labelled RNA using the miRNA Complete Labeling and Hyb Kit (Exiqon) in a hybridisation oven that was set at 55 °C and 20 r.p.m. for 20 h according to the manufacturer's instructions.
Possibly, these cells resided in the larger ducts during lactation where hardly any cells were YFP-labeled, in contrast to the almost complete labeling of the secretory cells in the alveoli (Additional file 7).
100 ng total RNA was used for Cy3-labeling of miRNAs by using the miRNA Complete Labeling and Hybridization Kit (Agilent Technologies) according to the manufacturer's instructions.
Labeled retinas were flat-mounted to confirm the complete labeling of the GCL.
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Be accompanied by ready-to-mount labels (permanent ink on acid free paper) with complete label data (see Specimen Data and Label Templates).
Once the shell is reasonably well cleaned and provided with a complete label, it can remain unaltered for years in a drawer or cabinet.
Finally, from the finiteness of the complete labeled simplex in S, the result follows.
Is a complete labeled sub-simplex able to resist the perturbation of functions or simplices?
After that, the seed's labels are propagated to all unlabeled regions by following some optimum criterion, such that a complete labeled image is constructed.
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