Your English writing platform
Discover LudwigExact(2)
Photos of the complete inserts were taken, coded and all cells were counted blind with ImageJ.
While many services offer individual pre-cut coupons, there are those whom sell complete inserts.
Similar(57)
How could a judge not agree that your spouse is a complete (insert description here) and side with you on all issues?
Using end reads only, 5,298 clones were complete insert sequenced to a high quality.
Sub-clones were sequenced to obtain 8× coverage of the complete insert.
To select candidate FLcDNAs for complete insert sequencing, we used a previously described bioinformatic pipeline for EST processing [ 11].
PUTs with a cDNA clone from a FLcDNA library were selected as candidates for complete insert sequencing.
From these libraries, we identified 8,127 cDNA candidate clones for complete insert sequencing, which resulted in 6,464 hq sequence-verified FLcDNA clones (Additional File 2).
The colonies shown to contain the complete insert were transferred to 5 ml LB broth with 50 μg/ml kanamycin and incubated at 37°C overnight.
For each PUT represented by multiple candidate clones after filtering, the clone with the longest 5' sequence was selected for complete insert sequencing.
Using a combination of end sequencing and primer walking, an additional 1,166 clones were complete insert sequenced, with an average insert size of 1,653 ± 447 bp, and requiring six end reads and 2.62 ± 1.51 internal primer reads per clone.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com