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All three now have achieved complete homogenization...that will produce exactly the same mildly tangy taste and thus go down exactly the same number of throats.... View Article Roger Angell, a senior editor and a staff writer, has contributed to The New Yorker since 1944, and became a fiction editor in 1956.
Both the solutions were ultrasonicated for 10 min to ensure complete homogenization.
Complete homogenization of type 2a inclusions in sample Gran-1b occurred at temperatures between 288 and 385°C, and there was a tight clustering of homogenization temperatures at 344 ± 15°C (Figure 7).
Moreover, AgNO3 is poured (0.75 M and 1 M) into the mixture, keeping the oxygen-free environment, and then the solution was stirred for 30 min (in dark) to reach a complete homogenization.
In the case of immobilized cells, the cell wet pellet correspondent to the mentioned OD was gently mixed with 2.8% sodium alginate and after complete homogenization, the mixture was added to 2.0% CaCl2 using sterile needles and a distance needle solution of 4 to 7 cm.
Six cycles were required for complete homogenization.
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For S. hystrix, tissue was airbrushed off the coral skeleton directly into RNA later Stabilization Solution (Qiagen, CA), followed by complete tissue homogenization.
Then, 20 ml of previously sterilized culture medium was poured onto the Petri dishes, tempered at 60°C, and gently stirred to complete the homogenization.
The brains were ground at low speed until homogeneous, then 5 additional strokes completed the homogenization.
The final wash was completed with homogenization buffer (100 mM KCl, 50 mM KH2PO4, 50 mM Tris, 5 mM MgCl2, 1 mM EDTA, and 1.8 mM ATP at pH 7.2) to dilute the presence of M9.
Frozen embryos were subsequently thawed on ice and pestle-homogenized in an initial 250 μL of TriZol (Life Technologies), to which 500 μL was added once homogenization was complete and the standard extraction protocol completed.
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