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Complete homogenisation of the tissue was done with a rotor-stator homogeniser TissueRuptor (Qiagen) and TCA precipitation of total protein followed.
We observed complete homogenisation at 1050°C for the volcanic ash; at 1200°C (Figure 2d) for the fine ATD; and at 1400°C for the coarse ATD.
These data demonstrate near complete homogenisation of both 18S and ITS1 sequences to a single ribotype.
The tissue was homogenised by RNase-free pestles and vortexed for 30 s to allow for complete homogenisation.
After 3 minutes (time required for a complete homogenisation of the water inside the system), the floodgate 1 was opened, the 2 and 3 were closed, 40 ml of water was collected with syringe T0 after what the 40 ml of the syringe 1 were injected into the system.
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lt is a perfect homogenisation of fats and liquids.
After completing the homogenisation, 200 μl of chloroform was added into the 2 ml tube.
The pyrene derivatives proved difficult to dissolve completely and a number of heating and cooling cycles were undertaken to ensure complete dissolution and homogenisation occurred.
The resulting data showed the progressive decrease of the density of the pellets, the preferential suction of the pellets and the final homogenisation at complete saturation.
Synovial tissues were homogenised as described previously [ 20] in 1 ml of complete Mini protease-inhibitor cocktail homogenisation buffer (Roche, Indianapolis, IN) on ice, followed by sonication for 30 seconds.
Hemispheres were separately homogenised mechanically in homogenisation buffer (140 mM NaCl; 20 mM Tris-HCl pH 7,6; 5 mM EDTA; 1× complete protease inhibitors) and extracts additionally sonicated for 30 s. Resulting extracts were centrifuged (6000 rpm) for 10 min at 4°C.
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