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The complete exchange of the solution within the chamber was completed within a few seconds.
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This rapid washout was not limited by the solution flow within the recording chamber, because only tens of seconds were required for complete exchange of the solutions.
The dye was allowed to remain on the cells for 60 s after cessation of the stimulus to permit complete compensatory endocytosis, and was subsequently removed during a 7 minute period with 8 complete exchanges of the solution.
An adsorbed layer remained after complete exchange of the polyelectrolyte/surfactant solution for aqueous NaCl.
Special care was taken to ensure complete exchange of the original buffer of the enzyme stock solution to phosphate buffer through repeated cycles of concentration and dilution using Vivaspin 6 ultrafiltration tubes (Vivascience, Hannover, Germany).
The development presented here allows complete exchange of the subphase of a spread or adsorbed film.
The large excess was used to guarantee complete exchange of the ligands.
All cultures were fed every 2 4 days by complete exchange of the medium.
Responses to toxin applications were recorded after at least 3 min of perfusion, sufficient time to allow complete exchange of solution in the recording chamber.
ADP and all other drugs were applied via a DAD-12 drug application device (Adams & List, Westbury, NY), which permits a complete exchange of solutions surrounding the cells under investigation within less than 100 ms (Boehm, 1999).
The time necessary for complete exchange of solution was measured with an open patch pipette by switching between Na+-rich and 20% Na+-rich solutions.
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