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The solid residue was obtained for complete dryness and preserved in desiccators.
After the growth, the sample was baked at 100°C for complete dryness.
Thereafter, PANI-NCC was then dried at 50 °C until complete dryness then weighed again.
H2SO4 and gradually kept at 130°C on a hot plate until complete dryness.
Ash was digested with 5 ml concentrated H2SO4 and gradually kept at 130 °C on hot plate until complete dryness.
The prepared samples were digested to complete dryness with an acid solution of (2 2 1 1) H2O-HF-HClO4-HNO3.
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The solution was set under room temperature for 10 minutes to allow the reaction to complete and then the solvent was evaporated to dryness under vacuum for 15 minutes.
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For quantification of the siderophore coprogen, the complete 50 ml culture supernatants were filtered and lyophilised to dryness.
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