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PAUSE POINT Samples can be incubated at 4°C overnight to complete dissolving the DNA.
After complete dissolving of starting materials and continuous mixing, the mixture was cooled to 0 ± 2 °C with the slow addition of precipitant 8% of ammonia (NH4OH).
Similarly, treatment of fibers with more that 1 m urea or GdnHCl led to their complete dissolving (Table 2).
The collected gallstones were dissolved by adding 10 mg of dry gallstone powder in 10 ml dimethylsulphoxide, and were vortexed rigorously for complete dissolving.
Complete dissolving of the beads was observed after 20 30 min. To remove excess alginate, cells were centrifuged, washed twice with sterile Hanks Salt Solution and resuspended in 2 ml modified cell culture medium (DMEM without phenol red, without ascorbic acid and only 3% FBS).
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Rate-limited growth proceeds only to this critical cell density, and then further growth is CO2 mass transfer limited until the final cell density at complete dissolved limiting nutrient consumption is achieved.
However, when influent p-cresol was at high levels (70 000 ppb), biotransformation of the other co-substrates in the mixture never ensued because p-cresol caused complete dissolved oxygen depletion.
When Saddam's regime collapsed in 2003, Paul Bremer stupidly oversaw the complete dissolve of the Baath party and the Iraqi army, possibly the only two institutions which could have kept the country intact had they been properly managed or restructured.
When the disks are completely dissolved, the fermentation is complete.
They benefit from the differences between members to co-create or co-execute specific tasks, and when the task is complete, they dissolve.
Either the Fenton reaction is not complete or dissolved ferrous iron is reacting with OH (eq. 6).
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