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Complete disaggregation of BODIPY FL-LPS by 2% SDS was measured.
The liver cells are then placed on a shaking incubator for 5 min at room temperature, followed by another 10 min of periodic pipetting to complete disaggregation.
In some cases, for example, the 1 9 gel of 4/ 7, the increase in excimer emission levels off at higher concentration of acetate, presumably as a result of complete disaggregation and, hence, full dissolution of the pyrenyl gelators.
First, it enabled complete disaggregation of the sources of data (which could be partially confounded by parent ratings, because parents who are distressed by marital conflict may also inflate their ratings of child ADHD symptoms).
Complete disaggregation typically occurred between 6 and 8 h of incubation in the case of rgHAD+ (Fig. 3c, bottom panel) and between 24 and 48 h in the case of rgHAD− (data not shown).These experiments indicated that the virus with hemadsorption-negative NA destroyed receptors on red blood cells more slowly than did its hemadsorption-positive counterpart.
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Homogeneous distribution of the filler in the polymer matrix as well as complete dispersion (disaggregation) of the particles are prerequisites for studying the influence of other parameters on the mechanical properties of composites.
Ocular tissues and cells require processing that should result in the complete solubilisation, disaggregation, reduction and denaturation of the proteins [ 23].
However, fibrils are usually resistant to agents employed to dissolve macromolecules, and the present protocol using trypsin, deoxycholate, and dithiothreitol for the proteomic analysis of the histologically abnormal areas may not necessarily have produced complete lysis and disaggregation, which suggests that there may be additional proteins that are enriched.
When disaggregation was complete, cells were centrifugated (5 min, 300g) to remove cell debris.
Fresh GIST samples were minced with scissors, washed twice in PBS, and incubated in a 0.25% solution of collagenase A (Roche Diagnostics, Meylan, France) at +37°C until disaggregation was complete.
After disaggregation and resuspension in complete medium, MCF-7 cells in 3-mL aliquots (≈ 1 × 10 cells) were seeded into 30-mm petri dishes containing 20-mm diameter coverslips (Sarstedt, UK).
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