Sentence examples for complete confluence from inspiring English sources

In contrast, on sample A, cells never reached complete confluence over the entire sample surface because of the higher pore volume.

Twelve hours after transfection, the RPE cells were lethally irradiated (9,000 rads), washed with FBS-supplemented RPMI 1640 medium and plated in 96-well flat-bottomed culture plates, using enough RPE cells to obtain complete confluence.

24 hours after complete confluence was reached cells were stimulated with TGF-α (50 ng/mL) for 48 hours or left non-stimulated for control before harvesting.

The initial redox ratio increase of HA hMSCs was accompanied by minimal proliferation to complete confluence, and not multiple cell layers, indicating that the cells reached a state of low activity prior to their delayed differentiation response.

After complete confluence, the Caco-2 cells were washed with sterile PBS (pH 6.7) thrice.

Each cell line was grown to complete confluence in appropriate media.

In all, 7 × 10 cells were seeded in a 96-well plate (covered with 0.2% gelatin in H2O) and incubated until complete cell confluence was reached.

Complete layer confluence was again achieved between d 18-21 of ALI and TEER was stable with values of about 300 Ω·cm.

All were propagated at 37°C in a 96% humid atmosphere with 4% CO2 in RPMI-1640 comedium medium to confluence to prepare RNA as for CHBE.

After 72 h, when SUM149 form 70-80% confluence complete culture media was replaced by serum free media containing different concentrations of each of recombinant MCP-1 and IL-8 (50, 100, 150 and 200 ηg/ml).

Cells were seeded at 5 × 104 cells/ml in 24 well plates (Corning® CellBIND® Surface cell culture flasks) for better attachment of cells and the cells grown to 70% confluence in complete EMEM for 48 h.