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This MPEC formulation minimizes the 2nd-order work expression subject to the set of constraints describing the complete complementarity system (in mixed static-kinematic variables) governing simultaneously the two adjacent equilibrium configurations, namely the current one and its neighboring state.
The regulatory process occurs posttranscriptionally and involves miRNA interaction with a target site in the mRNA that has partial or complete complementarity to the miRNA.
Translational suppression and mRNA degradation, modes by which mammalian miRNAs regulate gene expression, do not require complete complementarity between the miRNA and target.
While the dataset under study is rather large, there are few additional siRNAs that have complete complementarity to their target mRNAs.
Interestingly, simple sequence alignment analysis using the passenger or guide RNA strands of 5FU55 and the publicly available human RefSeq database did not identify a single target transcript with complete complementarity to these small RNA sequences.
In this study a simple sequence homology search indicated that the inserts identified through the two different selection assays did not recognise any sequences within the human transcriptome with complete complementarity and instead recognised between 7 and 20 different targets, using the criteria of 14 bases of contiguous homology.
Similar(28)
Another subgroup of antisense sRNAs is the cis-encoded sRNAs which are transcribed from the opposite strand of their target genes and regulate their target genes through complete complementarities [ 29].
Binding occurs with either perfect complementarity or, more often, imperfect complementarity [5].
The targeting depends on either complete sequence complementarity for inducing transcript degradation or partial sequence complementarity for translational repression.
Plant miRNAs pair with target mRNAs with nearly complete sequence complementarity and guide the cleavage of target mRNAs through the slicing activity of AGO1 (Mallory et al, 2004; Fahlgren & Carrington, 2010).
A significant advantage in using randomised RNAi libraries, over other nucleic acid-based libraries, in forward genetic approaches in mammalian cells would be the identification of 21 bases of complete sequence complementarity to the intracellular target RNA that is linked to the modified cellular phenotype.
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