Sentence examples for complete cassette from inspiring English sources
The reporters were subsequently transformed into haploid CEN.PK and integration of the complete cassette at the HO locus was confirmed by PCR amplification.
The 0.6 Kb RCAN1 cDNA was introduced in the EcoRI site of the β-globin exon 3. The complete cassette was sequenced with specific primers in an automated sequencer (Applied Biosystems 373A) to ensure the integrity of the transgene.
In the second transgenic line (P3-2) the complete cassette was successfully integrated (data not shown).
However, a part of a cassette or even a complete cassette may be also acquired via horizontal gene transfer [ 21].
Other transformants either had multiple copies of the cassette integrated (JP7.10, JP7.11, JP7.13, JP7.14 and JP7.15) or did not seem to insert the complete cassette into the genome (JP7.8) (Fig. S2 in the Supplementary Material).
The exemplar chosen is generally the first reported complete cassette, unless possible sequence errors or other problems have been identified, but the annotations in that GenBank entry may not be correct.
Only complete cassettes (with non-cassette flanking sequences) with defined orientation, were used.
Annotation of partial gene cassettes is subject to the same criteria as complete cassettes, but as distinguishing nucleotides may be missing, identification is potentially less accurate.
Finally, the complete cassettes for the expression of the CecA gene under the control of the glutelin promoters as KpnI- SacI fragments, and for the expression of the CecA-KDEL gene as KpnI fragments, were cloned into the pCAMBIA 1300 vector.
The complete dszB1A1C1-D1 cassette behaved as an attractive alternative — to the previously constructed recombinant dsz cassettes — for the conversion of DBT into 2HBP.
In the case of reporters bearing the complete gpt cassette, μ expression was undetectable in all eight independent replacements, indicating that the gpt cassette reduced expression by at least 80 fold, consistent with the results in the Appendix.
