Sentence examples for complete buffer from inspiring English sources
Let (Delta _{t}in mathbb {R}^{Ntimes N}) be an indicator matrix with binary elements {0,1} indicating the complete buffer contents of (bar {f}'_{t}) at time t.
This step was repeated three times to ensure complete buffer exchange.
Transfected CHO cells were collected and whole cell lysates were prepared in Complete Buffer (Roche Applied Science) according to the manufacturer's instructions.
Kinase assay buffer mix was prepared as follows: MgCl2 5 mM, CaCl2 300 μM, Tris-HCl pH 7.4 20 mM, PS 25 μlol/25 μl, PMA 1 μM or 1.6 μM was added separately to the complete buffer as needed.
However, the results demonstrated that any of the four insertions at this position would lead to the complete buffer insolubility as well as elimination of the native SH3 fold.
Total protein lysates were obtained after lysing the cells in RIPA complete buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 2 mM EDTA, 1% NP40 complemented with 0.1% SDS, 1 × EDTA-free protease inhibitor cocktail (Roche, Rotkreuz, Switzerland)).
Thereafter, worms were resuspended in S-complete buffer and diluted further to contain 10 15 worms per 10 μL.
The compounds were diluted 50-fold in complete assay buffer (assay buffer containing 5 mM dithiothreitol) to get a 2× final concentration and 2% DMSO.
Finally, mitochondrial pellets were lysed by adding 35 μl of complete mitochondria buffer, prepared by adding mitochondria buffer, protease inhibitor cocktail, and dithiothreitol, and by incubating on ice for 15 min. As a control, whole-protein extracts were obtained according to the standard procedure.
Immunoprecipitates were washed four times in a complete lysis buffer and finally heated in Laemmli buffer composed of glycerol 40% (Sigma), β-mercaptoethanol 0,35 M (Sigma), SDS 5% (Sigma), and Blue Bromophenol (Roche).
Immunoprecipitates were washed four times in complete lysis buffer and finally heated in LDS loading buffer 4X (Invitrogen) at 70°C for 10 minutes according to the manufacturer's protocol.
