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Model construction by the ProModII program included complete backbone and side chain building, loop building, verification of model quality, including packing, and subsequent energy minimization [33], [34] using the Gromos96 force field [35].
Complete backbone H and N resonance assignments were obtained for all residues except for amino acid E188 and the GSH residues remaining from the N-terminal His tag.
The fact that complete backbone assignments were obtained indicates that the P23T substitution did not cause global unfolding and/or gross aggregation.
CCBuilder is a web-based application that generates complete (backbone and side chain) atomistic models of heptad-based coiled coils in conformations specified by the user.
Summary: ADAPT-nuclear magnetic resonance (ADAPT-NMR) offers an automated approach to the concurrent acquisition and processing of protein NMR data with the goal of complete backbone and side chain assignments.
We have obtained near complete backbone assignments of C72S except for those of Glu, Pro and Val Asn (>91% of all the expected HN, Cα, Cβ, C' and N resonances).
Similar(51)
Their subsequent elimination resulted in a reduced-size binder with very similar affinity for NT, for which near-complete backbone assignments were achieved.
Near-complete backbone 1H/15N chemical shift assignments for full-length SNF were obtained (Figure S2).
This method capitalizes on both the critically important intact mass measurement of top-down MS to describe chain length and the almost-complete backbone fragmentation provided by N-terminally derived ions after UVPD to detect the ubiquitin linkage pattern, especially for homogeneously linked chains.
National Park Service receives grant money from Los Angeles County to purchase the properties that will complete the Backbone Trail.
These components were efficient in synthesizing a complete nonaketide backbone in E. coli.
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