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After complete addition of APiB-POSS, the reaction content was stirred for 20 h at ambient temperature.
After complete addition of the formaldehyde, the temperature of the reactants was elevated up to reflux temperature for 3 h.
The reaction mixture was vigorously stirred with a mechanical stirrer till the complete addition of the dianhydride to the flask kept at ice bath under inert atmosphere.
After complete addition of bromine, the reaction mixture was stirred vigorously at room temperature for further 2 h until evolution of hydrogen bromide gas ceased, then was poured onto ice.
After complete addition of the particles to the molten metal, the liquid composite was tilt-poured into the permanent steel mould, preheated at 250°C and then allowed to cool in the atmosphere.
After complete addition of bromine, the reaction mixture was stirred vigorously at room temperature for further 1 h till the release of hydrogen bromide gas ceased, then poured onto ice.
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In the absence of protein, metal loading of 140 μM zincon is complete upon addition of 140 μM Zn II).
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