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The mutations were made using the oligonucleotides listed below (mutations in lower case and underlined) and their reverse complements using two stage PCR as previously described [17].
Previous analyses of established and vegetatively propagating triploid Allium × cornutum (Clementi ex Visiani, 1842) suggested that it is one of the rare cases of allotriploid of triparental origin, and two parental taxa were suggested based on cytogenetic analyses of chromosome complements using GISH (genomic in situ hybridisation) [ 6].
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Leaching data were verified and complemented using chemical equilibrium calculations.
This can be complemented using information obtained by much more accurate sensors such as kinect.d.
The data are complemented using institution-level information on financial and logistical support for entrepreneurial activities.
These basis functions are complemented using a local, parameter dependent, weighted eigenvalue problem.
Then the BNA results have been calibrated against and complemented using the residual stress values measured using X-ray diffraction.
5448ΔdltA was complemented using plasmid pdltA, as described [13], creating 5448ΔdltA pdltA).
In contrast, in the absence of complement (using heat-inactivated serum) there was essentially no binding of HIV-1 to the cells.
Sequences obtained as described above were complemented using a classification-based search for Hymenogaster ITS sequences in GenBank (http://ncbi.nlm.nih.gov/).nih.gov/
These newly engineered mutant strains, PAO1-fadD1::FRT, PAO1-fadD2::FRT, and PAO1-ΔfadD2D1::FRT, were complemented using the relevant gene(s) on the miniCTX2 single copy integration vector as described previously [56].
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