Your English writing platform
Discover LudwigExact(2)
Interestingly the chromosomal copy of the tagatose-6-phosphate kinase lacC (SSUBM407_0883) is mutated in BM407, therefore the provision of an additional copy of this CDS on ICESsuSC84 complements the mutation.
In a previous paper, we isolated a novel gene, ltsA, that complements the mutation of a C. glutamicum lysozyme-sensitive mutant KY9714 [ 12].
Similar(58)
To complement the mutation, NIH-3T3 cells were stably transfected with the M115 open reading frame (gL-3T3).
Éric Déziel (personal communication) confirmed the mvfR phenotype of PA7, and complemented the mutation by adding a plasmid containing the wild-type mvfR gene from PAO1.
Taken together, these data demonstrated that the genomic fragment fully complemented the mutation.
We subsequently verified the phenotype-genotype relation by overexpression, reintroducing and complementing the mutation.
Likewise the Pch PRP8 His5-intein was able to complement the mutation in HIS3 in this genetic background.
However, multiple stocks from the Exelixis and Bloomington Stock Center deficiency collections did complement the mutation at this dose.
To complement the mutation data, and for a better overview of the activation status of the PI3K pathway, we investigated a set of previously published GSs.
After genotyping for the presence of the stm-dgh6 mutation, we could not distinguish the WT, stm-dgh6 heterozygotes and stm-dgh6 homozygote plants visually, confirming that pSTM STM-Venus can fully compSTM STM-Venusation.
Thus, dimerization of a subunit containing the inactivating mutation with a functional subunit can complement the mutation; this must occur either by lowering or by bypassing kinetic barriers in the ATPase or mechanical cycles of the mutant head.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com