Sentence examples for complements growth from inspiring English sources

Murine C2C12 myoblasts cells and C-26 colon adenocarcinoma (ATCC) were grown in Dulbecco's modified Eagle's medium (DMEM) with 10% fetal bovine serum plus standard complements (growth medium, GM).

The complemented single and double mutants fully restored growth on all FAs (Fig. 4), while empty vector miniCTX2 controls did not complement growth on the FAs (data not shown).

Although each amino acid mutation within yNhANT4 was sufficient to complement growth on nonfermentable carbon sources, the mutant yeasts that were originally recovered from EMS mutagenesis might have contained additional mutations outside of the yNhANT4 that also contributed to improved growth on nonfermentable carbon sources.

In an effort to expand the number of functional targets that can be studied using the Δtrk1Δtrk2 potassium transport deficient yeast system [27], we found that heterologous expression of the viral channel Kcv was able to complement growth under low potassium conditions [28].

Truncation of the G-patch domain of Pfa1 is unable to complement growth defect of cells lacking Pfa1 and Ltv1.

If the expression level is too low to fully complement growth, extra copies of VND7 under control of its native promoter could be introduced.

Second, the equivalent PD-like mutation in yeast VPS35 (D686N) is fully functional compared with the WT yeast protein in complementing growth phenotypes in VPS35 null yeast.

When the constitutive G. sulfurreducens acpP (GSU1604) promoter was used to express imcH in the pRK-Geo2 backbone (pImcH15), this construct fully complemented growth of the Δ imcH strain (Fig. 3A).

The suppressors G202K, G202Q, and G202I with their primary mutation I524K and I524Q were able to suppress the phenotypic defect caused by I524Q and I524K in terms of their ability to complement growth on glutathione.

Our preliminary results have shown that the recombinant yeast strain with LAT-1 does complement growth of EBY.VW4000 strain on D-glucose, indicating this protein can transport D-glucose also.

We employed yeast as a model to demonstrate that mutations equivalent to D620N in a highly conserved yeast ortholog of VPS35 (i.e. D686N) can normally complement growth phenotypes in Δ vps35 yeast thereby indicating that the D → N substitution at this highly conserved residue does not compromise VPS35 function.