Sentence examples for complementing strain from inspiring English sources

However, DS cultures of the complementing strain IH101 (MRS123) did show Ssp4 expression, thereby demonstrating that Spo0A is specifically required for Ssp4 production.

Therefore, to recover and propagate the lysA mutant, a complementing strain was constructed in which the lysA gene of phage Corndog is under control of the inducible acetamidase promoter[32].

Individual plaques recovered on the complementing strain were tested for dependence on complementation, and of 100 plaques examined, one was identified that does not grow on the control strain.

The cold temperature (4°C or −20°C) resistance of spores produced by wild-type, ssp4 null mutants, or complementing strains of those mutants were determined as described previously [12].

Complementing strains were created by introducing the plasmid expressing the wild-type C. rodentium NleL (pZP1661) or the catalytically-dead NleLC753S (pZP1664) into DBS792 generating DBS793 and DBS794 respectively.

Complementing strains were analysed by Southern hybridisation which confirmed, in the case of ΔhacA, the presence of the disrupted gene as well as the complementing plasmid (Fig.  3a, lanes 1 and 2).

Author's response: With the last analysis in the Results section, we tried to relate the phenotypic experimental differences of the argC complementing strains with its physico-chemical properties.

(A) Growth phenotype of E. coli fadR mutant strain K113 on minimal media with C10 as sole carbon source in comparison with those of the wild-type strain and the fadR complemented strain.

High challenge doses of either wild type or the dapAX complemented strain resulted in 100% lethality (data not shown).

Then pSET::C was electrotransformed into the ΔsalKR mutant to screen the complemented strain of ΔsalKR (namely CΔsalKR) on THB agar with double selection pressure of Spc and Cm.

DL and LJ made the GRA15GT1 complemented strain.