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In Figure 6G, it is rather surprising that the GY11 wt complementing line shows significantly higher induction of ASN1 and DIN10.
While in wrky33 leaves camalexin levels were significantly reduced in relation to wild type, wild type levels were restored in the complementing line.
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Instead, complementing lines of evidence support the view that important concept-driven processes (e.g., language comprehension) can draw on simulation of analog properties of the body and experience with the external world to ground meaning.
Using the DRB2-FlagHA complemented line and antibodies raised against DCL1, DCL3 and DCL4, we performed Flag IPs using an experimental set up very similar to the one we used previously to detect the DRB4/DCL4 interaction in vivo [ 50], but could not detect any interactions (Additional file 2: Figure S2a).
SG and IOI identified and characterized the Arabidopsis mutant lines; ACS, IOI, and BGA constructed plasmids; ACS transformed Arabidopsis and characterized complemented lines for growth and transient carbohydrate content; ACS and BGA prepared the manuscript; BGA did the uptake and exudation of labeled sugars, transcript analysis, and designed and coordinated the study.
Complementing this line of argument, a number of feminists argue that conceptualizing the self as a seamless whole has invidious social consequences.
Generation of epitope-tagged complementing lines.
We further tested genetic transmission of the tio-3 mutant allele in 15 complementing pMSP1-TIO lines and all of four complementing lines from pMSP2-TIO.
The LoxP/Cre RMCE strategy employed here to generate an Ad5 L4 complementing cell line could also be used to create cell lines to complement other viral vectors.
PRV mutants in the PRV Becker background are PRV 160 (Us9 null) [36]; PRV GS442 (gD null mutant with gD replaced by the GFP open reading frame; provided by G. Smith, Northwestern University), which was grown on a gD complementing cell line [37]; and PRV HF22A (gB null mutant), which was grown on a gB complementing cell line (LP64e3).
Cisco's gateway, which underwent a trial in Honolulu, functions similarly but is more hardware-based, complementing its line of network routing equipment and voice-over-IP telephony.
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