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Overnight cultures were diluted to 1 100 in Dubelcco's minimal Eagle medium high glucose (4500 mg.l−1) and grown for 3 h at 37°C without agitation complemented, when needed, with 1 mM isopropyl-β-D-thiogalactopyranoside for 30 min. Primers used in this study are listed in Table 2.
Similar(59)
The following search keywords and logic were adapted and complemented when necessary for each database: Diabetes AND (Gaps OR Barriers OR Needs Assessment OR Health Care Needs OR Challenges) AND (Family Practice OR Family Medicine OR General Practice).
"We understand construction when needed.
It was summoned when needed.
Remain neutral when needed.
Capitalise when needed.
Offer advice when needed.
Store when needed.
Offer help when needed.
(and Cure, when needed).
Reapply when needed!
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com