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Then the BNA results have been calibrated against and complemented using the residual stress values measured using X-ray diffraction.
These newly engineered mutant strains, PAO1-fadD1::FRT, PAO1-fadD2::FRT, and PAO1-ΔfadD2D1::FRT, were complemented using the relevant gene(s) on the miniCTX2 single copy integration vector as described previously [56].
JN24.6 was complemented using the same vector by performing cotransformation with the hygromycin resistance gene-containing plasmid pAN7.1.
This approach was complemented using the available protostome and Ciona EST data to identify putative N and C terminal ends of the protein (Additional file 1).
Minimal sample work-up was required and the results obtained by ELISA were further complemented using the LC-MS/MS method.
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Furthermore, an S. cerevisiae mutant that lacks the fatty acid desaturase gene OLE1 was complemented using constructs with the native (S.
We assessed each CNV-mediating HERV element for matches to the motif's position weight matrix and its reverse complement using the Biostrings package.
Mate pair reads were first reverse-complemented using the FASTX-toolkit [ 36].
These bottom-up approaches can, in principle, nicely be complemented by using the available genetic toolbox.
These searches were complemented by using the PubMed, LILACS, Scielo, and Medline electronic databases with no publication date- or language-based restrictions.
In parallel, the opportunity was undertaken to assess the effect of overexpressing each complementing gene using the strong P fdx promoter.
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