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Two oligonucleotides (forward 5′-CTCCTCAACTTCGATCACCGTCTC-3′ and Reverse 5′-GAGACGGTGATCGAAGTTGAGGAG-3′) complemented to each other were purchased from IGEbio and purified by HPLC.
However, this locus of >2.1 kb has regained a new position adjacent to DT6 in both M. avium subsp. paratuberculosis and M. avium subsp. hominissuis, albeit in opposite orientation (reverse complemented) to each other, as depicted in supplementary figure S2.
The two flanking sequences could be reversely complemented to each other.
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