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High challenge doses of either wild type or the dapAX complemented strain resulted in 100% lethality (data not shown).
DL and LJ made the GRA15GT1 complemented strain.
The FlpA mutant and FlpA complemented strain of C. jejuni were previously generated using standard techniques.
No band was detected in any of the protein fractions of the MtΔP27 mutant and MtΔP27::P55 complemented strain.
Exconjugants were selected on the basis of resistance to apramycin and kanamycin, yielding SB15003 as the Δ ltmK complemented strain.
The experiments of functional complementation with the complemented strain CΔsalKR showed clearly that the reintroduction of salKR into ΔsalKR restored its high pathogenicity.
The complemented strain had its cyst lysis capability restored, a phenotype that was enhanced for the lec-merodiploid bacteria (Fig. 5).
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Complementation did not restore invasiveness, and the re-complemented strain was significantly less invasive than the two other strains.
Immunostaining with EBA2 showed that ugtAΔ hyphae and conidiophores lacked Galf, which was restored in the AfglfB-complemented strain.
The phoP-complemented strain SO2-pSO5 largely restored the wild type growth pattern in PBMC.
Here, a significantly increased oxygen consumption in cells infected with S. aureus 8325-4 and the S. aureus ΔhemB hemB-complemented strain was detectable (Fig. 7A, B).
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