After the previous step, the complemented path-sets of the product are checked for inclusion between them.
For comparison of SAGE results with quantitative Real-time RT-PCR detection, two sets of primers and their antisense reverse-complemented sequences were derived from SAGE primer amplified napin 3'-termini (see above) and from assembled contigs of 8,977 B. napus ESTs aligning with an e-value cut-off of 1e-6 to A. thaliana 2S seed storage protein 1 (AT4G27140).
Using the fixed sets of isotone derivations, we establish characterizations of a chain, a distributive lattice, a modular lattice and a relatively pseudo-complemented lattice, respectively.
For this purpose, the localization of P27 was assessed in H37Rv as well as in the mutant MtΔP27, and in a set of complemented strains.
In order to test the susceptibility of a mutant of M. tuberculosis H37Rv in the P27-P55 operon to malachite green, sodium dodecyl sulfate, ethidium bromide, and first-line antituberculosis drugs, this strain together with the wild type strain and a set of complemented strains were cultivated in the presence and in the absence of these drugs.
Since the native SPL15n appeared to be ineffective at restoring the WT phenotype in the enhanced miR156b environment of sk156, another set of complemented sk156 lines was generated to express the miR156 -insensitive SPL15m under the control of the native SPL15 promoter (Table 1).
Now we discuss a characterization of relatively pseudo-complemented lattices by the fixed set of isotone derivations.
R ¯ denotes the set of reverse-complemented reads.
A relatively pseudo-complemented lattice (or Brouwerian lattice) is a lattice L in which, for any given elements a, b ∈ L, the set of all x ∈ L such that a ∧ x ≤ b contains a greatest element b : a, the relative pseudo-complement of a in b.
This hypothesis was tested in a small set of genes; three of these genes were not significantly different in terms of their expression in the three backgrounds, whereas one (At5g36910) was up-regulated by some 10 15 fold in the mutant compared with both the wild-type and complemented plants (not shown).
