Sentence examples for complemented lines from inspiring English sources
SG and IOI identified and characterized the Arabidopsis mutant lines; ACS, IOI, and BGA constructed plasmids; ACS transformed Arabidopsis and characterized complemented lines for growth and transient carbohydrate content; ACS and BGA prepared the manuscript; BGA did the uptake and exudation of labeled sugars, transcript analysis, and designed and coordinated the study.
Using the single gene complemented lines (+HASPB and +SHERP), we have shown that HASPB alone can complement the null phenotype observed with the KO parasites, with these parasites able to complete classical development and colonize the SV in late-stage infections.
However, in reaction to salt stress the SlIPT4 complemented plants displayed the same characteristics as WT in germination and survival assays, while both of the SlIPT3 complemented lines differed in their response to salinity.
Complemented lines Comp GFP 1, #2, and #3 resembled WT in AtPIP1 4 expression (Fig. 3a; Supplementary Fig. 6).
Gene structure of EGY1 and location of T-DNA insert (a), RT-PCR analysis of EGY1 expression in wild-type and egy1 leaves (b), phenotypes of wild-type, egy1 and egy1 complemented lines at 28 days after germination (c).
Transformation into the pgr7 mutant and screening of complemented lines was performed as described above.
Using the DRB2-FlagHA complemented line and antibodies raised against DCL1, DCL3 and DCL4, we performed Flag IPs using an experimental set up very similar to the one we used previously to detect the DRB4/DCL4 interaction in vivo [ 50], but could not detect any interactions (Additional file 2: Figure S2a).
Instead, complementing lines of evidence support the view that important concept-driven processes (e.g., language comprehension) can draw on simulation of analog properties of the body and experience with the external world to ground meaning.
In Figure 6G, it is rather surprising that the GY11 wt complementing line shows significantly higher induction of ASN1 and DIN10.
The complement lines for OsINV3 were generated by incorporation of this vector into the homozygous Tos17 mutant using Agrobacterium (EHA105) mediated transformation (Toki 1997).
Generation of epitope-tagged complementing lines.
