Sentence examples for complemented growth from inspiring English sources

When the constitutive G. sulfurreducens acpP (GSU1604) promoter was used to express imcH in the pRK-Geo2 backbone (pImcH15), this construct fully complemented growth of the Δ imcH strain (Fig. 3A).

We found that unlike the inward rectifier Kir2.1 (Minor et al, 1999; Chatelain et al, 2005), mammalian K2P2.1 (TREK-1) complemented growth of a potassium-uptake-deficient yeast strain SGY1528 (Tang et al, 1995) under the mild potassium limited conditions (1 mM KCl) but not under the most stringent complementation conditions (<1 mM KCl).

Although each amino acid mutation within yNhANT4 was sufficient to complement growth on nonfermentable carbon sources, the mutant yeasts that were originally recovered from EMS mutagenesis might have contained additional mutations outside of the yNhANT4 that also contributed to improved growth on nonfermentable carbon sources.

In an effort to expand the number of functional targets that can be studied using the Δtrk1Δtrk2 potassium transport deficient yeast system [27], we found that heterologous expression of the viral channel Kcv was able to complement growth under low potassium conditions [28].

Truncation of the G-patch domain of Pfa1 is unable to complement growth defect of cells lacking Pfa1 and Ltv1.

If the expression level is too low to fully complement growth, extra copies of VND7 under control of its native promoter could be introduced.

Second, the equivalent PD-like mutation in yeast VPS35 (D686N) is fully functional compared with the WT yeast protein in complementing growth phenotypes in VPS35 null yeast.

The suppressors G202K, G202Q, and G202I with their primary mutation I524K and I524Q were able to suppress the phenotypic defect caused by I524Q and I524K in terms of their ability to complement growth on glutathione.

We employed yeast as a model to demonstrate that mutations equivalent to D620N in a highly conserved yeast ortholog of VPS35 (i.e. D686N) can normally complement growth phenotypes in Δ vps35 yeast thereby indicating that the D → N substitution at this highly conserved residue does not compromise VPS35 function.

Various other Mrs2 proteins have been shown to partially complement growth defects of mrs2Δ cells, including a second CorA-related yeast protein, named Lpe10p, as well as a single human Mrs2p (13 , 15 16), some plant Mrs2 proteins and CorA of S. typhimurium.

For Ilv3 the truncated version Ilv3ΔN19 fully complemented the growth defect comparable to the wild-type enzyme and even the Ilv3ΔN19DE version mediated fast growth in the absence of valine.