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As expected [31], human Tpi1 complemented for the growth phenotype of Δtpi1.
To select potential P. fluorescens Pf0-1 clones that were complemented for their growth deficiency, all transconjugants were replica-plated to agarose plates containing 5 mM citrate and 10 mM β-valine as sole carbon and nitrogen sources.
SG and IOI identified and characterized the Arabidopsis mutant lines; ACS, IOI, and BGA constructed plasmids; ACS transformed Arabidopsis and characterized complemented lines for growth and transient carbohydrate content; ACS and BGA prepared the manuscript; BGA did the uptake and exudation of labeled sugars, transcript analysis, and designed and coordinated the study.
glutamicum shuttle vector pC2 [ 15], and the transformants were checked for temperature-sensitivity and lysozyme-sensitivity. Strains KY9704 and KY9706, which showed both lysozyme-sensitivity and temperature-sensitive growth, were complemented for lysozyme-sensitivity and temperature-sensitivity by pHLS4 like KY9714.
ES34+ and ES34− were tested for their ability to proliferate clonally in methylcellulose-based medium complemented with hematopoietic growth factors.
For Ilv3 the truncated version Ilv3ΔN19 fully complemented the growth defect comparable to the wild-type enzyme and even the Ilv3ΔN19DE version mediated fast growth in the absence of valine.
Ectopic expression of the At α-dox2 in a tomato mutant deficient for α-dox2 partially complemented the compromised growth phenotype, but deletion of At α-dox2 did not result in a growth phenotype [ 28].
While wild-type human HSF1 was unable to complement for the loss of yeast HSF, expression of the S303A, S307A or S303/307A HSF1 mutants allowed for human HSF1-dependent yeast growth (Figure 1A, B).
Human Tpi has 68% amino acid similarity to yeast Tpi (Blossum62) and complements for the growth phenotypes of Δtpi1 cells [14].
Complementation assays indicate that each GER mutant is able to complement an rpoBts mutant for growth at the non-permissive temperature, indicating that each GER RNAP derivative is sufficiently functional in transcription to support viability.
To complement our transcriptome analysis for growth on cellulose, we compared the growth patterns on different carbon sources in light and darkness for wild-type and an env1 non functional strain [ 13, 22], because of the strong effect of ENV1 on light dependent gene regulation.
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