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These cells were described and extensively characterized previously [ 18]. p65 cells complemented either with wild type p65, an empty plasmid as control (pTV), the acetylation-deficient double mutant K314/315R or the triple mutant KTR (K310/314/315R), were stimulated by TNFα for 3 hours and total RNA was isolated in three independent replicates from these cells.
This phenotype was observed in the Δ raaS mutant strains complemented either by M. tuberculosis or M. bovis BCG versions of raaS (survival values in both strains did not differ statistically [ P > 0.1, t test]), confirming that RaaS from either bacterium is capable of playing the same role.
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Technology takes a factor-augmenting form, and thus may complement either high or low skill workers.
As expected, nrx[313] failed to complement either of these other alleles, either with regard to NMJ neurexin staining or any of the major neurexin mutant phenotypes.
In total ~12,000 EMS-generated mutant lines were generated and screened for those that failed to complement either ATPalpha DTS1R1 or ATPalpha DTS2R3 null mutations for viability.
The identity of the cpr5 try-JC double mutants was confirmed by the failure of the double mutants to complement either parent mutant after crossing.
Complementing either strain with an integrative plasmid allowing expression of the WT Rv0561c gene [pNIP40b Rv0561c (Table S3)] restored synthesis of MK-9(H2) and depleted the pool of MK-9, clearly demonstrating that MSMEG1132 and Rv0561c encode proteins with analogous function.
Intriguingly, a deletion in the second half of M. smithii gene (the purE' region) removed the ability of that gene to complement either purE or purK deletions in E. coli [ 52], suggesting that it may actually be the PurE' that is involved in catalysis of both steps of this reaction, despite lower similarity of this second domain to known PurE enzymes.
Consistent with this, Alp7-L461A failed to complement either the hypersensitivity of the alp7Δ mutant to thiabendazole (TBZ; a microtubule depolymerizing drug) or growth at high temperature (supplementary Fig 1E online).
The touch-sensitive screen flips back nicely and is complemented on either side with 10 on-screen buttons for starting the built-in programs.
Much better agreement is achieved when at least three outgoing partons are complemented through either next-to-leading-order predictions or parton showers.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com